Education

• 2009-2013, Dual Bachelor’s degree in Molecular Science and Technology；
  • B.S. in Chemistry, Nankai University, 2009-2013
  • B.E. in Chemical engineering, Tianjin University, 2009-2013
• 2013-2016, Master’s degree in Chemical biology, supervised by Dr. Liu Hongxia in Prof. Jiang Yuyang’s group；
  • M.S. in Chemical Biology, Tsinghua University, 2013-2016
    Jiang’s group
• 2017-2021, Doctor of philosophy in Chemistry, supervisor: Prof. Cai Zongwei.
  • Ph.D in Chemistry, Hong Kong Baptist University, 2017-2021
    Cai Group
    

Occupation

• 2016-2017: Research Assistant
  • Hong Kong Baptist University
  • Duties included: Metabolomics and chemoproteomics
  • Supervisor: Professor Cai Zongwei
• 2021-2024: Postdoctoral Fellow
  
  • Department of Chemistry, The University of Hong Kong
  • Duties included: chemoproteomics
  • Supervisor: Professor Chi-Ming Che Che’s group
• 2024-present: Assistant Professor
  • Department of Toxicology, School of Public Health, The Sun Yat-Sen University

Fundings

• NSFC Young Scientist Fund，22406209, Chemical proteomics for investigating molecular mechanisms of hepatic toxicity induced by PPD-derived quinones, 2025-2027
• Basic scientific research start-up funds of SYSU, 2024-2026

Publications

Please check google scholar or researchgate

I.chemoproteomics for protein target identification

1. Shao, X., et al. Chemoproteomics for deconvolution of anticancer mechanism of gold complexes, 2024, in preparation.
2. Shao, X., et al. Integrative chemoproteomics reveals anticancer mechanisms of silver(I) targeting the proteasome regulatory complex, 2024, Chemical Science, online (Q1, IF=8.4).
3. Shao, X., Zhang, H., Yang, Z., Zhu, L., and Cai, Z. Quantitative profiling of protein-derived electrophilic cofactors in bacterial cells with a hydrazine-derived probe, Analytical Chemistry 2020, 92, 4484-4490. (Q1, IF=7.4, cited by 3)
4. Shao, X., Ji, F., Wang, Y., Zhu, L., Zhang, Z., Du, X., Chung, A. C. K., Hong, Y., Zhao, Q., and Cai, Z. Integrative chemical proteomics-metabolomics approach reveals Acaca/Acacb as direct molecular targets of PFOA, Analytical Chemistry 2018, 90, 11092-11098. (Q1, IF=7.4, cited by 25)

II.epitranscriptomics for investigating RNAs with noncanonical caps

1. Shao, X., et al. Response to the Comments on “Integrative chemoproteomics reveals anticancer mechanisms of silver(I) targeting proteasome regulatory complex”, 2023, Analytical Chemistry, accepted (Q1, IF=7.4).
2. Shao, X., et al. DpCoA tagSeq: barcoding dpCoA-capped RNA for direct nanopore sequencing via maleimide-thiol reaction, Analytical Chemistry 2023, 95, 11124–11131. (Q1, IF=7.4)
3. Shao, X., Zhang, H., Yang, Z., Zhong, H., Xia, Y., and Cai, Z. NAD tagSeq for transcriptome-wide identification and characterization of NAD+-capped RNAs, Nature Protocols 2020, 15, 2813-2836. (Q1, IF=14.8, cited by 11)
4. Hailei Zhang, Huan Zhong, Shoudong Zhang, Shao, X., Min Ni, Zongwei Cai, Xuemei Chen,and Yiji Xia. NAD tagSeq reveals that NAD+-capped RNAs are mostly produced from a large number of protein-coding genes in Arabidopsis. PNAS 2019.
5. Hailei Zhang, Huan Zhong, Xufeng Wang, Shoudong Zhang, Shao, X., Hao Hu, Zhiling Yu, Zongwei Cai, Xuemei Chen, and Yiji Xia. Use of NAD tagSeq II to identify growth phase-dependent alterations in E. coli RNA NAD+ capping, PNAS 2021.

III. others:

1. Shao, X., Gao, D., Chen, Y., Jin, F., Hu, G., Jiang, Y., and Liu, H. Development of a blood-brain barrier model in a membrane-based microchip for characterization of drug permeability and cytotoxicity for drug screening, Analytica Chimica Acta 2016, 934, 186-193. (Q1, IF=6.2, cited by 57)
2. Shao, X., Gao, D., Wang, Y., Jin, F., Wu Q., and Liu, H. Application of metabolomics to investigate the antitumor mechanism of flavopiridol in MCF-7 breast cancer cells. Journal of Chromatography B 2016, 1025 40–47. (Q1, IF=3, cited by 28)